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Ultraviolet-visible spectroscopy or ultraviolet-visible
spectrophotometry (UV-Vis or UV/Vis) refers to absorption
spectroscopy or reflectance spectroscopy in the ultraviolet-visible
spectral region. This means it uses light in the visible and
adjacent (near-UV and near-infrared (NIR)) ranges. The absorption
or reflectance in the visible range directly affects the perceived
color of the chemicals involved. In this region of the
electromagnetic spectrum, molecules undergo electronic transitions.
This technique is complementary to fluorescence spectroscopy, in
that fluorescence deals with transitions from the excited state to
the ground state, while absorption measures transitions from the
ground state to the excited state.
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