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Efficient DNA Purification Protocol for Fingerprinting of Sugarcane (Paperback) Loot Price: R1,432
Discovery Miles 14 320
Efficient DNA Purification Protocol for Fingerprinting of Sugarcane (Paperback): Md Munan Shaik, Mst Monira Khaton, Rms...
Efficient DNA Purification Protocol for Fingerprinting of Sugarcane (Paperback): Md Munan Shaik, Mst Monira Khaton, Rms...

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Efficient DNA Purification Protocol for Fingerprinting of Sugarcane (Paperback)

Md Munan Shaik, Mst Monira Khaton, Rms Shahnawas

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Loot Price R1,432 Discovery Miles 14 320 | Repayment Terms: R133 pm x 12*

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Sugarcane (Saccharum officinarum L.) is one of the leading crops in the world. Molecular characterization and molecular marker assisted selection in sugarcane breeding might be a tool for improvement of this crop can assist in increasing sugar production. Like many other plant species, sugarcane tissues contain high levels of polysaccharides and polyphenolic compounds, which present a major contamination problem during DNA purification. High-concentrations of polysaccharides, which co-purify with DNA in normal phenol-chloroform extractions and polyphenols covalently bind to DNA making it useless for most research applications. An rapid, efficient and easy DNA purification procedure for sugarcane is an urgent need in genome mapping and marker assisted selection (MAS) programs. In the present investigation an efficient and easy method was developed for isolation of high quality DNA from meristem cylinder in sugarcane. With the method developed high quality DNA isolation was possible without use of liquid nitrogen, tissue homogenizer and using a simple micro-centrifuge. Isolated DNA well performed for PCR amplification and DNA Fingerprinting using RAPD markers.

General

Imprint: Lap Lambert Academic Publishing
Country of origin: Germany
Release date: May 2011
First published: May 2011
Authors: Md Munan Shaik • Mst Monira Khaton • Rms Shahnawas
Dimensions: 229 x 152 x 7mm (L x W x T)
Format: Paperback - Trade
Pages: 116
ISBN-13: 978-3-8443-9462-7
Barcode: 9783844394627
Categories: Promotions
Books > Science & Mathematics > Biology, life sciences
Books > Science & Mathematics > Biology, life sciences > General
LSN: 3-8443-9462-1

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