Sugarcane (Saccharum officinarum L.) is one of the leading crops in
the world. Molecular characterization and molecular marker assisted
selection in sugarcane breeding might be a tool for improvement of
this crop can assist in increasing sugar production. Like many
other plant species, sugarcane tissues contain high levels of
polysaccharides and polyphenolic compounds, which present a major
contamination problem during DNA purification. High-concentrations
of polysaccharides, which co-purify with DNA in normal
phenol-chloroform extractions and polyphenols covalently bind to
DNA making it useless for most research applications. An rapid,
efficient and easy DNA purification procedure for sugarcane is an
urgent need in genome mapping and marker assisted selection (MAS)
programs. In the present investigation an efficient and easy method
was developed for isolation of high quality DNA from meristem
cylinder in sugarcane. With the method developed high quality DNA
isolation was possible without use of liquid nitrogen, tissue
homogenizer and using a simple micro-centrifuge. Isolated DNA well
performed for PCR amplification and DNA Fingerprinting using RAPD
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