Protein Phosphorylation - A Practical Approach (Book)

Reversible phosphorylation is one of the major mechanisms of controlli ng protein activity in all eukaryotic cells. This new edition of Prote in Phosphorylation: A Practical Approach provides a comprehensive desc ription of current methods used to study protein phosphorylation and t he kinases and phosphatases which catalyse it. It includes protocols f or studying phosphorylation in intact cells; analysis of signal transd uction pathways, kinase specificity, and kinase interactions; assay an d purification of kinases and phosphatases; and identification of subs trates. Also covered are cloning and expression protocols and advice o n the crystallization of kinases and phosphatases. Protein Phosphoryla tion: A Practical Approach 2e will therefore be of great value to any researcher investigating aspects of reversible protein phosphrylation.