Collected Papers from the Research Laboratory Volume 1 (Paperback)

This historic book may have numerous typos and missing text. Purchasers can usually download a free scanned copy of the original book (without typos) from the publisher. Not indexed. Not illustrated. 1913 edition. Excerpt: ...an agglu-: tinin titre of 1 in 2,100 from sera produced in exactly the same manner, the lowest titre being 1 in 100 and the highest 1 in 4,000 by serum H with culture H. Cross agglutinations gave much higher reactions with sera and organisms of the opposite classification, as the accompanying chart shows..... The first four sera agglutinate their corresponding four cultures very uniformly, while they do not agglutinate the micrococcus scarcely at all; and again, the last three anti-sera, namely, M, H, and W, do not agglutinate the four staphylococcic cultures, while they do agglutinate very uniformly their own cultures in the same class. The chart represents 49 combinations between 1 cultures and the 7 anti-sera. agglutinations of the sera on cultures of like and opposite strains. The chart also shows that the cultures of the micrococcus are homologous with sera produced by strains of organisms from the same class only, and heterogeneous when sera from strains of Staphylococcus pyogenes albus are used. These results would seem to indicate that in this micrococcus we have an organism which differs in the ability to produce an anti-serum of high agglutinin titre and that its anti-serum is not specific to cultures of Staphylococcus pyogenes albus. Sera produced with different strains of Staphylococcus pyogenes albus are somewhat lower in agglutinin, both to the cultures of their origin and to the cultures of the micrococcus. ''-rki A limited number of attempts were made to produce a satisfactory antigen, by means of which we hoped to establish the relation between this organism and the Staphylococcus pyogenes albus by means of the complement fixation test. The unsatisfactory tests made indicate that it may be used with the micrococcus, ...