Molecular Biology and Genetic Engineering (Electronic book text)

PART IMolecular Biology1. Molecular Biology and Genetic Engineering Definition, History and Scope2. Chemistry of the Cell: 1. Micromolecules (Sugars, Fatty Acids, Amino Acids, Nucleotides and Lipids)Sugars (Carbohydrates)3. Chemistry of the Cell .2. Macromolecules (Nucleic Acids; Proteins and Polysaccharides)Covalent and Weak Non-covalent Bonds4. Chemistry of the Gene: Synthesis, Modification and Repair of DNADNA Replication: General Features5. Organisation of Genetic Material 1. Packaging of DNA as Nucleosomes in EukaryotesTechniques Leading to NucleosomeDiscovery6. Organization of Genetic Material2. Repetitive and Unique DNA Sequences7. Organization of Genetic Material:3. Split Genes, Overlapping Genes, Pseudogenes and Cryptic GenesSplit Genes or .Interrupted Genes8. Multigene Families in Eukaryotes9. Organization of Mitochondrialand Chloroplast Genomes10. The Genetic Code11. Protein Synthesis Apparatus Ribosome, Transfer RNA and Aminoacyl-tRNA SynthetasesRibosome12. Expression of Gene . Protein Synthesis1. Transcription in Prokaryotes and Eukaryotes13. Expression of Gene: Protein Synthesis: 2. RNA Processing (RNA Splicing, RNA Editing and Ribozymes)Polyadenylation of mRNA in ProkaryotesAddition of Cap (m7G) and Tail (Poly A)for mRNA in Eukaryotes14. Expression of Gene: Protein Synthesis: 3. Synthesis and Transport of Proteins (Prokaryotes and Eukaryotes)Formation of Aminoacyl tRNA15. Regulation of Gene Expression:1. Operon Circuits in Bacteria and Other Prokaryotes16. Regulation of Gene Expression .2. Circuits for Lytic Cycle and Lysogeny in Bacteriophages17. Regulation of Gene Expression3. A Variety of Mechanisms in Eukaryotes(Including Cell Receptors and Cell Signalling)PART IIGenetic Engineering18. Recombinant DNA and Gene Cloning1. Cloning and Expression Vectors19. Recombinant DNA and Gene Cloning2. Chimeric DNA, Molecular Probes and Gene Libraries20. Polymerase Chain Reaction (PCR)and Gene Amplification21. Isolation, Sequencing and Synthesis of Genes 22. Proteins: Separation, Purification and Identification 23. Immunotechnology 1. B-Cells, Antibodies, Interferons and Vaccines24. Immunotechnology2. T-Cell Receptors and MHC Restriction25. Immunotechnology 3. Hybridoma and Monoclonal Antibodies (mAbs)Hybridoma Technology and the Productionof Monoclonal Antibodies26. Transfection Methods and Transgenic Animals 27. Animal and Human Genomics: Molecular Maps and Genome SequencesMolecular Markers28. Biotechnology in Medicine: l.Vaccines, Diagnostics and ForensicsAnimal and Human Health Care29. Biotechnology in Medicine2. Gene TherapyHuman Diseases Targeted for GeneTherapyVectors and Other Delivery Systems forGene Therapy30. Biotechnology in Medicine: 3. Pharmacogenetics / Pharmacogenomics and Personalized MedicinePhannacogenetics and Personalized31. Plant Cell and Tissue Culture'Production and Uses of Haploids32. Gene Transfer Methods in Plants33. Transgenic Plants . Genetically Modified (GM) Crops and Floricultural Plants34. Plant Genomics:35. Genetically Engineered Microbes (GEMs)and Microbial GenomicsReferences