Abel's Laboratory Handbook of Bacteriology (Paperback)

This historic book may have numerous typos and missing text. Purchasers can download a free scanned copy of the original book (without typos) from the publisher. Not indexed. Not illustrated. 1907 Excerpt: ...hours at 37 C. This is a distinction from B. coli, which causes the medium to become red, coagulates it, and produces gas. If the medium contains 1 % glucose in place of the lactose, B. ty. produces redness and coagulation within twentyfour hours at 37 C. This is a distinction from the dysentery bacillus which does not produce redness within twenty-four hours, and only produces coagulation later. Certain micro-organisms can only be distinguished from B. ty. by application of all the above ten tests. For instance, B. faecalis alkaligenes is only with certainty differentiated by test 5 and in similar manner by test 10 (it forms alkali); B. dysenteriae by test 1 (absence of motility), by test 10, and by the further actions referred to under B. dysenteriae (p. 126). Moreover the Paratyphoid bacilli which give rise to diseases clinically resembling typhoid, of which two forms have been differentiated, are in many respects similar to typhoid. The rare Paratyphoid A forms circular colonies without markings in 2, the commoner Paratyphoid B similar colonies but thick white and when young iridescent, stains potato brown, slowly decolorizes milk with production of alkali, renders litmus whey after 8-10 days alkaline. Both form gas and fluorescence in test 9, and produce coagulation in litmus glucose nutrose solution. But although no micro-organisms other than typhoid have been found to answer all the ten tests in the manner of that organism, the surest means of diagnosis is by 11. The Serum Reaction, which has two forms, viz. (a) bacteriolysis in the animal body and (b) agglutination. (a) Bacteriolysis in the animal body (the socalled Pfeiffer's Reaction). Principle. B. ty., when mixed with the serum of an animal highly immunized against it, and injected into the peri...