Recombinant DNA Principles and Methodologies (Hardcover)

This comprehensive yet balanced work emphasizes the principles and rationale underlying recombinant DNA methodology while furnishing a general understanding of the experimental protocols-suggesting flexible approaches to resolving particular molecular necessities that are easily adaptable to readers' specific applications. Features summary tables presenting at-a-glance information on practices of recombinant DNA methodologies! Recombinant DNA Principles and Methodologies discusses basic and advanced topics requisite to the employment of recombinant DNA technology, such as -plasmid biology -nucleic acid biochemistry -restriction enzymes -cloning strategies -gel electrophoresis -southern and northern blotting -preparation of probes -phage lambda biology -cosmids and genome analysis -cloned gene expression -polymerase chain reaction -conventional and automated DNA sequencing -site-directed mutagenesis -and more! Elucidating the material with over 2250 edifying references, equations, drawings, and photographs, this state-of-the-art resource is a valuable hands-on guide for molecular and cell biologists, biochemists, bioprocess technologists, applied and industrial microbiologists, virologists, geneticists, chemical engineers, and upper-level undergraduate and graduate students in these disciplines.