Proceedings of the Society for Experimental Biology and Medicine Volume 12 (Paperback)

This historic book may have numerous typos and missing text. Purchasers can download a free scanned copy of the original book (without typos) from the publisher. Not indexed. Not illustrated. 1915 Excerpt: ...kept guinea-pig blood and dog blood several days in plasma with a minimum of citrate and then revived exsanguinated animals with it.2 Do red cells survive longer in the preservative solutions we have devised than in such plasma citrate? There is no doubt that human cells and sheep cells do. In plasma-citrate these rapidly disintegrate. Rabbit's cells last longer but in their case we have had better results with the preservatives than with a plasma-Ringer's-citrate. On the other hand, it is possible that an optimum plasma-citrate medium has not yet been found. Our most recent experiments demonstrate that the blood of some species when allowed to flow directly into a large excess of a preservative fluid in which citrate is present can be kept for long periods. And since the cells soon settle out, and practically all the preserving fluid can be pipetted off previous to the employment of the blood for injection this constitutes a great simplification of method. 1 Locke used one tenth of one per cent, of dextrose in the solution which bears his name. This amount of sugar is far too little for any preservative effect on the red blood cell. R. Weil, Jour. Am. Med. Assn., 1915, LXIV, 425. The Respiratory Quotient In Diabetes. 125 75 (1oo7) The respiratory quotient in diabetes. By Graham Lusk. From the Physiological Laboratory of the Cornell University Medical College, New York City. Oxidation of protein in the body really consists in the destruction of a great variety of amino-acids. When glucose arises from protein in diabetes the oxidation is different from the normal. When the D: N ratio is 3.65 the respiratory quotient for protein falls from 0.801 to 0.634. From Osborne's analyses of meat protein, recalculated on the basis of Osborne's own determination of the...