Methods of Morbid Histology and Clinical Pathology (Paperback)

This historic book may have numerous typos and missing text. Purchasers can download a free scanned copy of the original book (without typos) from the publisher. Not indexed. Not illustrated. 1905 Excerpt: ...The nuclei are blue to violet; the granules, red-violet. The granules may be also well demonstrated by staining in a saturated solution of 50 per cent thionin for 10 minutes and differentiating in 50 per cent alcohol. Then dry and pass through xylol, and mount in balsam. Loeffler's methylene blue, or methylene blue in 70 per cent alcohol, or Ehrlich's tri-acid mixture, or HeidenhainBiondi mixture, with differentiation in weak acetic acid solution, or a 1 per cent solution of polychrome methylene blue (1-12 hours) with differentiation in equal parts of glycerin and ether, also give good results. (For the details of staining mast cells elsewhere consult Unna, Monat. prakt. Derm. Bd. 13, 19, and 33.) The mast cells are best stained in tissue sections (see Chapter II.). The fine basophile (8) granules occur chiefly in mononuclear transitional leucocytes, and may be stained with a saturated watery solution of methylene blue for 10-20 minutes, then washed in water, dried, and mounted in balsam. 3. Neutrophile (e) granules--Ehrlich's tri-acid solution: --Saturated watery solution of orange G. 120 c.c. acid fuchsin 80 c.c., methyl green 100 c.c. Glycerin....50 c.c. Distilled water 300 c.c. Absolute alcohol.180 c.c. The solution should not be used until it is 6 months old; it should be neither shaken nor filtered, the required stain being removed by a pipette. Stain for 5-10 minutes. Aronson and Philipp's modified tri-acid solution: --Saturated watery solution of orange G. 55 c.c. rubin S 50 ex. Distilled water.. 100 c.c. Absolute alcohol..50 cc. Add to the above solution: --Saturated watery solution of methyl green 65 c.c. Distilled water...50 c.c. Absolute alcohol...12 c.c. After standing for 2 weeks the stain is ready for use...