Manual of Practical Normal Histology (Paperback)

This historic book may have numerous typos, missing text or index. Purchasers can download a free scanned copy of the original book (without typos) from the publisher. 1886. Not illustrated. Excerpt: ... APPENDIX. Page Io. Section-Cutting with the Microtome.--Although it is very important for every worker in normal Histology to be able to cut thin sections with the free hand, it is desirable in many cases to make use of an instrument called the Microtome for this purpose; since, when many sections are to be cut, much time is thereby saved, and sections can be made much thinner and smoother. One of the best instruments is that devised by Prof. R. Thoma, of Heidelberg, and known as Thoma's Microtome. This instrument is made of three sizes, and the intermediate or largest size is most useful. This can be imported from the maker, Rudolph Jung, of Heidelberg, Germany; or it can be obtained from the Prang Educational Co., 7 Park Street, Boston, Mass., or of Meyrowitz Bros., corner of Fourth Ave. and Twenty-third St., N. Y. The method of using the instrument need not be described here, since with the instrument at hand any worker will readily make out for himself the necessary procedure. The instrument and mode of using are described in your. Royal Microscopical Sac, vol. Hi., p. 298, 1883. The Freezing Microtome.--For many purposes it is desirable to study thin sections of fresh tissues which have not been subjected to the action of hardening agents. Such sections are best prepared with a so-called freezing microtome, by means of which, by the action usually of a spray of ether or rhigolene, small pieces of tissue may in a few seconds be made hard and easily cut off in thin slices. The freezing microtome of Thoma is one of the simplest and cheapest, and can be obtained as above. A thin bit of the fresh tissue, not more than 2-3 mm. thick, is placed on the metal plate, and a spray of ether from an ordinary two-bulbed atomizer being directed against the lower side of th...